Table 1.
Primers sequences for RT-PCR amplification
| Gene name and symbol | Sequence (5′–3′) | Amplicon size (bp) | TM | |
|---|---|---|---|---|
| F | R | |||
| GAPDH |
F:5′-AAGCTCATTTCCTGGTATGACAACG-3′ R:5′-TCTTCCTCTTGTGCTCTTGCTGG-3′ |
126 | 60.3 | 61.1 |
| ERBB 2 |
F: 5′-TGTGACTGCCTGTCCCTACAA-3′ R: 5′-CCAGACCATAGCACACTCGG-3′ |
152 | 62.8 | 62 |
| ERBB 3 |
F: 5′-GACCCAGGTCTACGATGGGAA-3′ R: 5′-GTGAGCTGAGTCAAGCGGAG-3′ |
100 | 62.7 | 62.5 |
| CASPAS 8 |
F: 5′-GACAGAGCTTCTTCGAGACAC-3′ R: 5′-GCTCGGGCATACAGGCAAAT-3′ |
116 | 60.1 | 63 |
| CASPAS 9 |
F: 5′-CTCAGACCAGAGATTCGCAAAC-3′ R: 5′-GCATTTCCCCTCAAACTCTCAA-3′ |
116 | 60.9 | 60.5 |
| BAX |
F: 5′-CCCGAGAGGTCTTTTTCCGAG-3′ R: 5′- CCAGCCCATGATGGTTCTGAT-3′ |
155 | 62.1 | 61.9 |
| BCL-2 |
F: 5′- GGTGGGGTCATGTGTGTGG-3′ R: 5′-CGGTTCAGGTACTCAGTCATCC-3′ |
99 | 62.6 | 61.8 |
| BCL-XL |
F: 5′- GAGCTGGTGGTTGACTTTCTC-3′ R: 5′-TCCATCTCCGATTCAGTCCCT-3′ |
119 | 60.6 | 61.9 |