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. 2020 May 8;11:798. doi: 10.3389/fimmu.2020.00798

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Copyright © 2020 Nono, Lutz and Brehm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EmACT promotes IL-10 release by CD4⁺ T-cells in vitro. CD4⁺CD25^– T-cells freshly isolated from C57BL/6 mice were stimulated with CD3/CD28 antibodies in the presence of supernatants from pSecTag2-transfected (Control) or pSecTag2-emact-transfected HEK cells (rEmACT). After 72 h, the T-cells supernatants were collected and probed for IL-10 concentration by Elisa. Horizontal bars represent the mean from two independent experiments with T-cells from two different isolations individually activated in the presence of HEK supernatant batches from two different transfections. *p < 0.05.