FIGURE 1.

Experimental layout indicating the timeline where 28 trays of tall fescue (TF) and 28 trays of perennial ryegrass (RG) plants associated with the endophyte Epichloë sp. FaTG-3, strain AR501, were originally grown within a glasshouse until they were two weeks old (top white box). At week 0, eight trays (four trays of TF and four trays of RG) were removed for harvesting, while the remaining 48 trays (24 trays of TF and 24 trays of RG) were transferred to either a warm (25/16°C: day/night) growth chamber, symbolized with an orange box, or a cool (12/6°C: day/night) chamber, symbolized with a blue box, for three weeks. At week 3, 16 trays (four trays of TF from the cool environment, four trays of RG from the cool environment, four trays of TF from the warm environment and four trays of RG from the warm environment) were removed for harvesting, while the remaining 32 trays were either left in the same environment or transferred to a different environment (either a warm or cool temperature regime depending on the environmental regime they had previously been subjected to) for a further three weeks. At week 6, the remaining 32 trays of TF and RG from all environmental regimes were removed for harvesting.