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. 2020 Apr 18;17:975–985. doi: 10.1016/j.omtm.2020.04.008

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© 2020 The Authors.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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HTSFcAng(1-7) Exhibited Specific Overexpression in Pulmonary Endothelial Cells and Hypoxic Inducibility in Hypoxia

(A) Diagram of the constructed HTSFcAng(1-7) and HTSFc. (B) Effects of HTSFcAng(1-7) or HTSFc transfection on the expression of angiotensin-(1-7) in A549 cells, HEK293 cells, NIH 3T3 cells, pulmonary artery smooth muscle cells (PASMCs), pulmonary microendothelial cells (PMVECs), rat peripheral blood neutrophils, monocytes, NR8383 cells, and primary bone mesenchymal stem cells (MSCs). (C and D) Effect of oxygen concentration on the expression of angiotensin-(1-7) (C) and the increment rate of the expression of angiotensin-(1-7) (D) in PMVECs transfected with HTSFcAng(1-7). ∗p < 0.05. Data are represented as mean ± SD from three replicated experiments (B and C, respectively).