Figure 2.

Effects of the Condition Medium from PMVECs Transfected with HTSFcAng(1-7) on Hypoxia-Induced Proliferation of Primary PASMCs

PASMC proliferation was measured by the 3-(4, 5-dimethylthiazal-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay. (A) Effects of oxygen concentration on the proliferation of rat primary PASMCs. (B) Inhibition effects of the condition medium from PMVECs transfected with HTSFcAng(1-7) on the proliferation of PASMCs cultured in normoxia (20% O₂) and in hypoxia (10%, 5%, and 1% O₂). (C and D) Concentration (v/v)-dependent inhibition effects of the condition medium, from the PMVECs transfected with HTSFcAng(1-7), on the proliferation of PASMCs cultured in 10% O₂ concentration. CM, condition medium. ∗p < 0.05. Data are represented as mean ± SD from three replicated experiments in each panel.