Figure 3.

Effects of the Condition Medium from PMVECs Transfected with HTSFcAng(1-7) on the Constriction and Relaxation of the Isolated Pulmonary Artery Rings in Hypoxia

(A) The representative curve for the effects of acute hypoxia on phenylephrine-precontracted pulmonary artery rings. Acute hypoxia resulted in vascular biphasic contractions: an initial transient contraction, then a transient relaxation (maximum dilation), and finally a delayed, sustained contraction (phase II constriction). (B–E) The representative curve for the effect of the condition medium, which was from PMVECs transfected with HTSFcAng(1-7) (B and C) or HTSFc (D and E), on the artery ring in hypoxia (% O₂) with (C and E) or without (B and D Mas receptor antagonist A-779 (1 μM). (F and G) The summarized data of maximum vasodilation (F) and phase II vasoconstriction and phase II vasodilation (G). CM, condition medium. *p < 0.05 versus control and HTSFcAng(1-7). Data are represented as mean ± SD, n = 6.