FIGURE 1.

Genetic labeling of astrocytes and EdU pulse-chase labeling of proliferating cells during postnatal development. (A) Experimental paradigm for genetic labeling of astrocytes and EdU pulse-chase labeling of proliferating cells at different time points postnatally; specific time points for 4-OHT and EdU injections are indicated. (B) Representative images of astrocyte labeling with the tdT reporter in slc1a3-CreERT⁺Ai14-tdT^f/+ mice and EdU labeling of proliferating cells in P7 brain; magnified images show cortical astrocytes. Magenta arrow, tdT⁺EdU^– cell; white arrow, tdT^–EdU⁺ cell; yellow arrow, tdT⁺EdU⁺ cell. Scale bar: 1 mm (i–iii), 10 μm (i’–iii’).