FIGURE 3.

Astrocyte proliferation activity in cortex and hypothalamus determined by genetic labeling of astrocytes and Ki67 immunostaining. (A) Experimental strategy for genetic labeling of astrocytes and Ki-67 staining of proliferating cells at different postnatal time points. (B) Representative images of astrocyte labeling with the tdT reporter in Slc1a3-CreERT⁺Ai14-tdT^f/+ mice and Ki-67 labeling of actively proliferating cells in P7 brain; magnified images show cortical astrocytes. Scale bars: 1 mm (left panel), 30 μm (magnified images). Percentage of proliferating astrocytes (100 × tdT⁺Ki-67⁺/tdT⁺) in cortex and hypothalamus at P7 (C) and P14/P15 (D); n = 3 images/mouse from 3–4 individual mice per region/time point. p-values determined by unpaired two-tailed t-test.