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. 2020 May 8;14:435. doi: 10.3389/fnins.2020.00435

FIGURE 4.

FIGURE 4

Morphological maturation of subcortical astrocytes during postnatal development. (A) Representative confocal and 3D images of astrocytes from hypothalamus of eaat2-tdT mice. Scale bar: 20 μm; (B) cumulative frequency curve of astroglial domain size in hypothalamus at different postnatal developmental time points (P7, P14, and P26). n = 60 astrocytes/group from multiple mice; (C) representative confocal and 3D images of astrocytes from different brain regions of eaat2-tdT mice at P30. Scale bar: 20 μm; (D) cumulative frequency curve of astroglial domain size in these brain regions. The insert bar graph represents the average astroglial domain size (one-way ANOVA, significant differences between the means, p = 0.002, F(3,145) = 5.220). n = 50–84 astroglia/group from multiple mice; p-value in the figure determined by post hoc Tukey’s test. (E) Cumulative frequency curve of thalamic astroglial domain size in VGluT1–/–tdT+ and VGluT1+/+tdT+ mice. n = 70 astrocytes/group from multiple mice; n.s., not significant.