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. 2020 May 8;11:683. doi: 10.3389/fmicb.2020.00683

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Copyright © 2020 Kivistik, Knobloch, Käiro, Tammert, Kisand, Hildebrandt and Herlemann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nuclear marker internal transcribed spacer 2 (ITS2) sequence maximum likelihood tree based on 398 sequence columns without outgroup. A sequence from Theodoxus fluviatilis transcriptome analysis (EST library) was used as reference (marked in bold). Freshwater samples are indicated in italics. The scale bar indicates a 0.5% sequence difference. J29, J25, J24, J12 with 1% sequence difference J6, J11, J4, J3 with 0.5% sequence difference.