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. 2020 May 8;11:636. doi: 10.3389/fmicb.2020.00636

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Copyright © 2020 Witt, Andreotti, Busch, Neubert, Reinert, Tomaso and Meierhofer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protocol comparisons between iST and TCA sample preparation methods and autoclavation vs. ethanol inactivation to detect F. tularensis strains. (A) The identified protein groups and the unique peptides without missed cleavages (MC, B) were plotted for two indicated strains in technical duplicates. Furthermore, the overlap of unique peptides between both protocols for the same sample and inactivation method (C) and the overlap of unique peptides for the iST protocol for both inactivation methods within the same sample (D) are shown.