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. 2020 May 11;55:102766. doi: 10.1016/j.ebiom.2020.102766

Fig. 6.

Fig 6

GLP-1 levels in ileum and energy expenditure were decreased significantly in HF-OP group. (a) Representative immunofluorescent images showing TGR5(red), GLP-1(green) in the ileum tissue of N, HF-OP and HF-OR groups. Scale bars, 50 μm. GLP-1 positive cells count was expressed as counts per mm2 of mucosal area. Five fields of view were chosen randomly per sample to calculate mean count and area, values are presented as mean ± SD. n = 5 per group. The count and area were analysed using image J software. *p<0•05 and **p<0•01 (unpaired Student's t-test). (b) The GLP-1 precursor Glucagon and Tgr5 mRNA in ileum tissue were measured using real-time PCR assay. Data are presented as the mean ± SD. *p<0•05 and **p<0•01 (unpaired Student's t-test). n = 4 per group. (c) GLP-1 secretion was detected in STC-1 and NCI-H716 cells using ELISA with treatment of BAs, and data were obtained from 3 independent experiments. *p<0•05 and **p<0•01 (unpaired Student's t-test). (d) Representative UCP1 immunostaining of BAT sections from the three groups. Scale bars, 50 μm. (e) Quantification of UCP1 immunostaining average optical density. Data are presented as the mean ± SD. n = 3 per group. *p<0•05 and **p<0•01 (unpaired Student's t-test). (f) The expression of UCP1 (n = 8 per group) and PGC-1α (n = 7 per group) in BAT were detected by western blot. Data are presented as the mean ± SD. **p<0•01 (unpaired Student's t-test).