Table 1.
Specimens and sequences used for the molecular phylogenetic analyses (i.e., the ITS dataset and the 28S + TEF1-α and 28S + RPB1 combined datasets). Sequences newly generated for this study are highlighted in bold.
| Taxon | Voucher No. | Locality | ITS | 28S | TEF1-α | RPB1 |
|---|---|---|---|---|---|---|
| Afroboletus luteolus | 00-436 | Africa | - | KF030238 | KF030397 | - |
| Afrocastellanoa ivoryana | Arora 126 | Mukuvisi, Zimbabwe | - | KX685721 | KX685715 | - |
| Australopilus palumanus | REH 6791 | Queensland, Australia | - | JX889650 | JX889691 | - |
| Austroboletus fusisporus | HKAS75207 | China | - | JX889720 | JX889718 | - |
| Austroboletus gracilis | 112/96 | MA, USA | - | DQ534624 | KF030425 | KF030358 |
| Austroboletus mutabilis | BRI AQ0795793 | Queensland, Australia | - | KP242263 | - | KP242078 |
| Austroboletus subvirens | BRI AQ0794171 | Queensland, Australia | - | KP242227 | - | KP242045 |
| Baorangia bicolor | MB07-001 | NY, USA | - | KF030246 | - | KF030370 |
| Baorangia pseudocalopus | HKAS 63607 | China | - | KY418895 | - | KJ184564 |
| HKAS 75739 | China | - | KJ184558 | - | KF030369 | |
| HKAS 75081 | Nanhua County, Yunnan Prov., China | - | KF112356 | KF112168 | - | |
| Boletus aereus | REH 8721 | Humboldt County, California, USA | - | KF030339 | - | KF030377 |
| Boletus edulis | Be3 | Bavaria, Germany | - | KF030282 | GU187682 | - |
| BD380 | Colorado, USA | - | HQ161848 | - | - | |
| Boletus pulchriceps | DS4514 | Chiricahua Mnts, AZ, USA | - | KF030261 | KF030409 | - |
| Boletus rufomaculatus | 4414 | Chestnut Ridge Park, NY, USA | - | KF030248 | KF030406 | KF030369 |
| Boletus semigastroideus | PBM3076 | Auckland, New Zealand | - | KF030352 | KF030430 | - |
| Boletus variipes var. fagicola | A.H. Smith 4249 | Cheboygan Co, Michigan, USA | - | JQ327014 | JQ327017 | - |
| Borofutus dhakanus | HKAS 73792 | Bangladesh | - | JQ928617 | JQ928575 | - |
| Bothia castanella | MB 03-053 | MA, USA | DQ867110 | DQ867117 | KF030421 | KF030382 |
| NY28003 | NY, USA | DQ867111 | - | - | - | |
| NY8669 | NY, USA | DQ867112 | - | - | - | |
| NY28002 | NY, USA | DQ867113 | - | - | - | |
| MB 03-067 | MA, USA | DQ867114 | - | - | - | |
| Bothia fujianensis | HKAS 82693 | Fujian Prov., China | KM269196 | - | - | - |
| HKAS 82694 | Fujian Prov., China | KM269195 | KM269193 | KM272860 | - | |
| Buchwaldoboletus lignicola | HKAS 76674 | Yichun, Heilongjiang Prov., China | - | KF112350 | KF112277 | KF112642 |
| Butyriboletus appendiculatus | Bap1 | Bavaria, Germany | - | AF456837 | JQ327025 | - |
| Butyriboletus roseoflavus | HKAS 54099 | China | - | JX290184 | - | KF739741 |
| Caloboletus firmus | MB 06-060 | Chestnut Ridge Park, NY, USA | - | KF030278 | KF030408 | KF030368 |
| Caloboletus inedulis | MB 06-044 | Erie Co., NY, USA | - | JQ327013 | JQ327020 | KF030362 |
| Chalciporus piperatus | MB 04-001 | Rutland State Park, MA, USA | - | DQ534648 | GU187690 | GU187453 |
| Fistulinella prunicolor | REH 9502 | Fraser Island, Queensland, Australia | - | JX889648 | JX889690 | - |
| Gymnogaster boletoides | REH 9455 | Cooloola, Queensland, Australia | - | JX889673 | JX889683 | - |
| Gyrodon lividus | Gl1 | Bavaria, Germany | - | AF098378 | GU187701 | - |
| Harrya chromapes | HKAS 50527 | Dêqên, Yunnan Prov., China | - | KF112437 | KF112270 | - |
| Heliogaster columellifer | KPM-NC 23012 | Odawara, Kanagawa Pref., Japan | - | KX685724 | KX685718 | - |
| Hourangia cheoi | HKAS 52269 | China | - | KF112385 | KF112286 | - |
| Hourangia microcarpa | HKAS 83763 (Wu1324) | China | - | KP136945 | KP136923 | - |
| Hourangia nigropunctata | HKAS 76657 | China | - | KF112388 | KF112287 | - |
| Hourangia sp. | HKAS 68178 | China | - | KF112453 | KF112301 | - |
| Imleria badia | xb2 | Bavaria, Germany | - | KF030357 | KF030422 | - |
| S-F119691 | Sweden | - | KJ806971 | KJ806969 | - | |
| Imleria obscurebrunnea | HKAS 52557 | Ning’er, Yunnan Prov., China | - | KF112374 | KF112190 | KC215225 |
| Lanmaoa angustispora | HKAS 74759 | China | - | KM605140 | - | KM605167 |
| Lanmaoa asiatica | HKAS 63592 | Heqing, Yunnan Prov., China | - | KM605142 | KM605152 | KM605163 |
| Lanmaoa carminipes | MB 06-061 | Erie Co., NY, USA | - | JQ327001 | JQ327022 | - |
| Leccinellum aff. griseum | KPM-NC 17831 | Hyogo Pref., Japan | - | JN378508 | JN378449 | - |
| Leccinellum cremeum | HKAS 90639 | China | - | - | - | KT990936 |
| Leccinellum crocipodium | KPM-NC 18041 | Yazu-cho, Tottori Pref., Japan | - | KC552053 | KC552094 | - |
| Leccinum monticola | HKAS 76669 | China | - | KF112443 | - | KF112592 |
| Leccinum scabrum | KPM-NC 17840 | Burn O’ Vat, Scotland, UK | - | JN378515 | JN378455 | - |
| Leccinum versipelle | KPM-NC 17833 | Scotland, UK | - | JN378514 | JN378454 | - |
| Neoboletus magnificus | HKAS 74939 | Baoshan, Yunnan Prov., China | - | KF112320 | KF112148 | - |
| Nigroboletus roseonigrescens | GDGM 43238 | Guangdong Prov., China | - | KT220588 | KT220595 | - |
| Octaviania decimae | KPM-NC 17763 | Mt. Hiei, Kyoto Pref., Japan | - | JN378465 | JN378409 | - |
| Octaviania kobayasii | KPM-NC 17785 | Mt. Kasuga, Nara Pref., Japan | - | JN378478 | JN378420 | - |
| Octaviania nonae | KPM-NC 17748 | Amami-oshima, Kagoshima Pref., Japan | - | JN378459 | JN378403 | - |
| Octaviania tasmanica | MEL2341996 | Tasmania, Australia | - | JN378495 | JN378436 | - |
| Octaviania yaeyamaensis | KPM-NC 17819 | Ishigaki Isl., Okinawa Pref., Japan | - | JN378491 | JN378432 | - |
| Paragyrodon sphaerosporus | MB06-066 | Iowa, USA | - | GU187593 | - | - |
| Paxillus vernalis | MB062 (CUW) | China | - | AY645059 | - | - |
| Phylloboletellus chloephorus | XAL3388 | Veracruz, Municipio Coatepec, El Grande, Mexico | - | DQ534658 | - | - |
| Phylloporopsis boletinoides | JBSD127411 | Jarabacoa, Dominican Republic | MH571675 | MH571711 | MH588312 | - |
| JBSD127412 | Jarabacoa, Dominican Republic | MH571676 | MH571712 | MH588313 | - | |
| JBSD127413 | Jarabacoa, Dominican Republic | MH571677 | MH571713 | MH588314 | - | |
| JBSD127414 | Jarabacoa, Dominican Republic | MH571678 | MH571714 | MH588315 | - | |
| FLAS-F-60407 | Putnam County, Florida, USA | MG845193 | - | - | - | |
| FLAS-F-60413 | Putnam County, Florida, USA | MG845194 | - | - | - | |
| FLAS-F-61158 | Putnam County, Florida, USA | MH211774 | - | - | - | |
| Farid 617 (USF 296126) | Tampa, Florida, USA | MG817716 | MG817715 | - | MG820263 | |
| CORT014483 | Mountain Pine Ridge, Belize | MH571679 | MH571715 | MH588316 | - | |
| CORT010991 | Kountze, Texas, USA | - | MH571716 | MH588317 | - | |
| F1118420 | Sarasota, Florida, USA | MH571680 | MH571717 | - | - | |
| Phylloporus attenuatus | HKAS 76168 (holotype) | Tangail, Bangladesh | - | NG_059569 | KR094791 | - |
| Phylloporus bellus | HKAS 56763 | Yunnan, SW China | - | JQ967196 | JQ967153 | - |
| Phylloporus brunneiceps | HKAS 56903 | Yunnan, SW China | - | JQ967198 | JQ967155 | - |
| Phylloporus catenulatus | HKAS 76157 | Bangladesh | - | KR094779 | KR094789 | - |
| Phylloporus gajari | HKAS 76158 | Gazipur, Bangladesh | - | KR231697 | KR231695 | - |
| Phylloporus imbricatus | HKAS 54647 | Yunnan, SW China | - | JQ967202 | JQ967159 | - |
| HKAS 68642 | China | - | KF112398 | KF112299 | - | |
| Phylloporus leucomycelinus | HKAS 74678 | eastern USA | - | JQ967206 | JQ967163 | - |
| Phylloporus luxiensis | HKAS 57036 | Yunnan, SW China | - | JQ967207 | JQ967164 | - |
| HKAS 75077 | China | - | KF112490 | KF112298 | KF112636 | |
| Phylloporus maculatus | HKAS 56683 | Yunnan, SW China | - | JQ967210 | JQ967167 | - |
| Phylloporus pachycystidiatus | HKAS 54540 | Yunnan, SW China | - | JQ967211 | JQ967168 | - |
| Phylloporus parvisporus | HKAS 54768 | Yunnan SW China | - | JQ967214 | JQ967171 | - |
| Phylloporus pelletieri | Pp1 | Austria | - | AF456818 | JQ327036 | KF030390 |
| Phylloporus pelletieri | Q7199c | Slovakia | - | JQ003668 | - | - |
| K 128205 | England, UK | - | JQ967215 | - | - | |
| Phylloporus rhodoxanthus | SAR 89.457 | eastern USA | - | U11925 | - | - |
| MAN075 | eastern USA | - | JQ003674 | - | - | |
| REH8714 | eastern USA | - | JQ003675 | - | - | |
| MAN099 | eastern USA | - | JQ003676 | - | - | |
| JLM1808 | eastern USA | - | JQ003688 | - | - | |
| BD374 | - | HQ161851 | - | HQ161820 | ||
| Phylloporus rubeolus | HKAS 52573 | Yunnan, SW China | - | JQ967216 | JQ967172 | - |
| Phylloporus rubrosquamosus | HKAS 54542 | Yunnan, SW China | - | JQ967217 | JQ967173 | - |
| HKAS 52552 | China | - | KF112391 | KF112289 | - | |
| Phylloporus rufescens | HKAS 59722 | Hainan, southern China | - | JQ967220 | JQ967176 | - |
| Phylloporus yunnanensis | HKAS 52225 | Yunnan, SW China | - | JQ967222 | JQ967178 | - |
| Phylloporus sp. | HKAS 74679 | Hunan, central China | - | JQ967228 | JQ967184 | - |
| HKAS 74680 | Fujian, SE China | - | JQ967229 | JQ967185 | - | |
| HKAS 74681 | Hainan, southern China | - | JQ967227 | JQ967183 | - | |
| HKAS 74682 | Yunnan, SW China | - | JQ967230 | JQ967186 | - | |
| HKAS 74683 | Yunnan, SW China | - | JQ967231 | JQ967187 | - | |
| HKAS 74684 | Fujian, SE China | - | JQ967232 | JQ967188 | - | |
| HKAS 74685 | Yunnan, SW China | - | JQ967233 | JQ967189 | - | |
| HKAS 74687 | Yunnan, SW China | - | JQ967235 | JQ967190 | - | |
| HKAS 74688 | Yunnan, SW China | - | JQ967236 | JQ967191 | - | |
| HKAS 74689 | Hainan, southern China | - | JQ967237 | JQ967192 | - | |
| Porphyrellus brunneus | REH 9527 | Fraser Island, QLD, Australia | - | JX889647 | JX889689 | - |
| Porphyrellus porphyrosporus | KPM-NC 22667 | Mt. Tarumae, Hokkaido, Japan | KX685713 | KX685722 | KX685716 | - |
| KPM-NC 25017 | Rishiri Island, Hokkaido, Japan | KX685714 | KX685723 | KX685717 | - | |
| MB 97-023 | Walhalla, Bavaria, Germany | DQ534563 | DQ534643 | GU187734 | - | |
| HKAS 76771 | Yanbian, Jilin Prov., China | - | KF112482 | KF112243 | - | |
| Porphyrellus sp. | HKAS 53366 | Sanming, Fujian Prov., China | - | KF112480 | KF112241 | - |
| HKAS 75078 | Chuxiong, Yunnan Prov., China | - | KF112481 | KF112242 | - | |
| Pseudoboletus parasiticus | Xps1 | Bavaria, Germany | - | AF050646 | KF030443 | - |
| Retiboletus fuscus | HKAS59460 | Yunnan Prov., China | - | JQ928626 | JQ928580 | - |
| Retiboletus griseus | Both sn | NY, USA | - | KF030308 | KF030414 | KF030373 |
| Rossbeevera griseovelutina | TNS-F-36989 | Hyogo, Japan | - | KC552031 | KC552076 | - |
| Rossbeevera vittatispora | MEL2128491 | NSW, Australia | - | KX685725 | KX685719 | - |
| Royoungia boletoides | Trappe 27456 | NSW, Australia | - | JX889655 | JX889696 | - |
| Rubroboletus rhodosanguineus | 4252 | Chestnut Ridge Park, NY, USA | - | KF030252 | KF030412 | - |
| Solioccasus polychromus | J. Trappe 15399 | Australia | JX888459 | - | - | - |
| REH 9417 | Queensland, Australia | - | JQ287642 | JQ287644 | - | |
| Spongiforma thailandica | DED 7873 | Thailand | - | EU685108 | KF030436 | - |
| Strobilomyces strobilaceus (as S. floccopus) | Sf1 | Bavaria, Germany | - | DQ534626 | JQ327037 | - |
| Suillellus amygdalinus | 112605ba | Mendocino Co., CA, USA | - | JQ326996 | JQ327024 | - |
| Sutorius aff. eximius | HKAS 52672 | Kunming, Yunnan Prov. CHINA | - | KF112399 | KF112207 | - |
| Sutorius eximius | REH 8594 | Jardin de Dota, Costa Rica | - | JQ327008 | JQ327027 | - |
| Turmalinea persicina | KPM-NC 18001 | Iwakura Kyoto Pref., Japan | - | KC552038 | KC552082 | - |
| Tylopilus alpinus | HKAS 55438 | China | - | KF112404 | - | KF112538 |
| Tylopilus ballouii | Osmundson 1198 | Thailand | - | EU430740 | - | EU434340 |
| REH 9467 | Fraser Island, Queensland, Australia | - | JX889676 | JX889686 | - | |
| Tylopilus felleus | AT2001011 | Uppsala, Sweden | - | JQ326993 | JQ327015 | |
| HKAS 90203 | China | - | KT990545 | - | KT990913 | |
| MCVE98230 | Italy | JF908787 | - | - | - | |
| Tylopilus ferrugineus | MB 06-053 | Erie Co., NY, USA | - | JQ326994 | JQ327016 | - |
| Tylopilus microsporus (T. neofelleus) | HKAS 59661 | Yunnan Prov., China | - | KF112450 | KF112225 | - |
| Tylopilus otsuensis | HKAS 53401 | Chenzhou, Hunan, China | - | KF112449 | KF112224 | - |
| Tylopilus plumbeoviolaceus | MB06-056 | NY, USA | - | KF030350 | KF030439 | - |
| Veloporphyrellus alpinus | HKAS 57490 | Yunnan Prov., China | - | KF112380 | KF112209 | KF112555 |
| Xerocomellus chrysenteron | Xch1 | Bavaria, Germany | - | AF050647 | KF030415 | - |
| Xerocomellus zelleri | REH 8724 | Humbolt Co., CA, USA | - | KF030271 | KF030416 | KF030366 |
| Xerocomus magniporus | HKAS 59820 | Yunnan, SW China | - | JQ678699 | JQ967195 | - |
| Xerocomus perplexus | MB00-005 | USA | - | JQ003702 | KF030438 | - |
| Xerocomus subtomentosus | KM167686 | England, U.K. | - | KC215222 | - | - |
| Xs1 | Bavaria, Germany | - | AF139716 | JQ327035 | KF030391 | |
| Zangia citrina | HKAS 52684 | China | - | HQ326941 | - | - |
| Zangia erythrocephala | HKAS 75046 | Nujiang, Yunnan, Prov., China | - | KF112414 | KF112269 | - |
| Zangia roseola | HKAS 52661 | China | - | JQ928623 | - | JQ928595 |
| Uncultured Boletaceae | clone 47C_G1_H9 | Jonathan Dickinson State Park, Hobe Sound, Florida, USA | KX899732 | - | - | - |
| clone 4C_G2_C3 | Big Lagoon Start Park, Pensacola, FL, USA | KX899785 | - | - | - |