Figure 5.

Defining the Transcriptome of the First Functional HSCs

(A) Plot of hscScore against CD27 MFI for 85 CD31^hiSSC^locKit^hiGata2^medCD27^+N cells. Majority of ICGS2 cells (black) found in the CD27^med and higher hscScore range and ICGS1 cells (red) within the full CD27 MFI and mostly low hscScore range. ICGS3 cells are indicated in gray.

(B) Ly6a expression was plotted on the SPRING visualization of (Bi) 85 CD31^hiSSC^locKit^hiGata2^medCD27^+N cells, (Bii) 71 CD27^med cells (CD27^hi in green), and (Biii) 14 CD27^hi cells (CD27^med in green) within HC1 (gray).

(C) Replotting of 85-cell heatmap in Figure S4 (maintaining the same order and position) with subset of ICGS output (Figure S5) for the 23 Ly6a⁺CD27^med cells belonging to HC1 (L2.7+L2.8). 3 gene clusters (RowCluster) and 2 cell clusters (ICGS1 and ICGS2) can be defined. HscScore, log₁₀ of the CD27 index-sorting (protein) levels and Louvain identity (L2.7, L2.8) are also depicted.