REAGENT OR RESOURCE	SOURCE	IDENTIFIER
Antibodies
polyclonal rabbit anti-Septin 7	IBL	Cat#18991; RRID: AB_10705434
monoclonal mouse anti-β-actin	Sigma-Aldrich	Cat#A5316; RRID: AB_476743
monoclonal mouse anti-CDC42	BD Transduction Laboratories	Cat#610929; RRID: AB_398244
monoclonal mouse anti-ArpC3 p21-Arc Clone26	BD Transduction Laboratories	Cat#612234; RRID: AB_399557
monoclonal mouse anti-RAC1 clone 23A8	Abcam	Cat# ab33186; RRID: AB_777598
anti-mouse horseradish peroxidase-conjugated	Bio-Rad	Cat#170-6516; RRID: AB_11125547
anti-rabbit horseradish peroxidase-conjugated	Bio-Rad	Cat#170-6515; RRID: AB_11125142
Bacterial strains
Shigella flexneri (WT) 5a M90T-Sm virulence plasmid pWR100 (GenBank#CM001474.1, GenBank#AL391753.1)	Philippe Sansonetti John R. Rohde	(Onodera et al., 2012)
S. flexneri icsB- (non-polar icsB mutant)	Claude Parsot	N/A
S. flexneri icsB-(compl.) (icsB- mutant carrying pUC8::icsB-ipgA expressing IcsB and its chaperone IpgA)	Claude Parsot	N/A
S. flexneri icsB-(C306A) (icsB- mutant carrying pUC8::icsB(C306A)-ipgA expressing enzymatic inactive IcsB-C306A and its chaperone IpgA)	This work	N/A
S. flexneri (WT) mCherry (expressing mCherry and Afa-I (E. coli pIL22))	Guy Tran Van Nhieu	N/A
S. flexneri (WT) (expressing Afa-I (afaE gene) from E. coli)	John R. Rohde	(Labigne-Roussel et al., 1984)
S. flexneri (WT) DsRed (expressing dsRed and Afa-I)	Yuen-Yan Chang	N/A
S. flexneri ΔicsA (ΔicsA::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔipgB1 (ΔipgB1::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔipgD (ΔipgD::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔipaJ (ΔipaJ::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔospG (ΔospG::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔospC1 (ΔospC1::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔvirA (ΔvirA::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
S. flexneri ΔipaH7.8 (ΔipaH7.8::tetRA expressing Afa-I)	John R. Rohde	(Sidik et al., 2014)
Escherichia coli DH5α	Thermo Fisher Scientific	Cat#18265017
Escherichia coli InvA (expressing Yersinia invasin A)	Guy Tran Van Nhieu	N/A
Salmonella enterica serovar Typhimurium (S. Typhimurium) SL1344 / pGG2-dsRed (WT)	ATCC	ATCC SL1344
Chemicals, Peptides, and Recombinant Proteins
X-tremeGENE 9 DNA transfection reagent	Roche	Cat#6365779001
Lipofectamine RNAiMAX	Invitrogen	Cat#13778030
DAPI	Thermo Fisher Scientific	Cat#D1306
Alexa Fluor 647 Phalloidin	Thermo Fisher	Cat#A22287
Paraformaldehyde 16%	Electron Microscopy Sciences	Cat#15710
Ampicillin	Sigma-Aldrich	Cat#A9393
Kanamycin	Sigma-Aldrich	Cat#K1377
Isopropyl-β-D-thiogalactoside (IPTG)	Sigma-Aldrich	Cat#10724815001
Poly-L-lysine	Sigma-Aldrich	Cat#P4707
Agarose	Invitrogen	Cat#16500-500
Dulbecco’s Modified Eagle’s Medium, High Glucose, GlutaMax	Thermo Fisher Scientific	Cat#10566016
Fetal Bovine Serum	Sigma-Aldrich	Cat#F7524
DPBS	Thermo Fisher Scientific	Cat#14190144
MEM Non-Essential Amino Acids Solution	Thermo Fisher Scientific	Cat#11140050
HEPES	Thermo Fisher Scientific	Cat#15630080
0.05% Trypsin-EDTA	Thermo Fisher Scientific	Cat#25300054
ProLong Gold Antifade Mountant	ThermoFisher Scientific	Cat#P10144
FluoroBrite DMEM	ThermoFisher Scientific	Cat#A1896701
Complete Protease Inhibitor	Roche	Cat#11836170001
RIPA buffer	ThermoFisher Scientific	Cat#89901
NuPAGE 4-12% Bis-Tris Protein Gels	Thermo Fisher Scientific	Cat#NP0321BOX
Micro BCA™ Protein Assay Kit	Thermo Fisher Scientific	Cat#23235
Trans-Blot® Turbo RTA Mini Nitrocellulose Transfer Kit	Bio-Rad	Cat#1704270
Trans-Blot© Turbo Transfer System	Bio-Rad	Cat#1704150
SuperSignal West Pico PLUS Chemiluminescent Substrate	Thermo Scientific	Cat#34577
QIAprep Spin Miniprep Kit	QIAGEN	Cat# 27106
Q5® Site Directed mutagenesis kit	New Englands Biolabs	Cat#E0554S
Inhibitors
Cytochalasin D	Enzo Life Sciences Inc	Cat#BML-T109-0001
SMIFH2	Sigma	Cat#S4826
Y-27632	BD biosciences	Cat#562822
NSC23766	Calbiochem	Cat#553502
(±)-Blebbistatin	BioVision	Cat#BV-2405-5
Wortmannin	Enzo Life Sciences Inc	Cat#BML-ST415-0001
CK666	Sigma	Cat#SML0006
ETH1864	Tocris Bioscience	Cat#3872
ML141	Tocris Bioscience	Cat#71203-35-5
Experimental Models: Cell Lines
HeLa Human cervix carcinoma epithelial cell line clone CCL-2	ATCC	Cat#11033106
Caco-2 TC7 cells	Philippe Sansonetti	N/A
HeLa cells stably expressing galectin-3-mOrange	Patricia Latour-Lambert	N/A
Oligonucleotides
ON-TARGETplus Non-targeting Control Pool	Dharmacon	Cat#D-001810-10-05
SEPT7 siRNA	Ambion	Cat#s2743
ON-TARGETplus SMARTpool CDC42	Dharmacon	Cat#L-005057-00-0005
ON-TARGETplus SMARTpool ARPC3	Dharmacon	Cat#L-005284-00-0005
ON-TARGETplus SMARTpool RAC1	Dharmacon	Cat#L-003560-00-0005
Recombinant DNA
pmCherryC1-Arp3	Christian Merrifield	(Taylor et al., 2011) Addgene Cat#27682
pmCherryC1- Cortactin	Christian Merrifield	(Taylor et al., 2011) Addgene Cat#27676
pmCherryC1-Cofilin	Christian Merrifield	(Taylor et al., 2011) Addgene Cat#27687
pEYFP-C1-Villin	Sylvie Robine	(Revenu et al., 2007)
pEGFP-C1-TOCA-1	Jennifer Gallop	N/A
pGFP-Cortactin	Kenneth Yamada	Addgene Cat#50728
mEmerald-N-WASP-C-18	Michael Davidson	Addgene Cat#54199
mEmerald-Coronin1B	Michael Davidson	Addgene Cat#54050
pmCherry-C1-WIP	Anna Huttenlocher	Addgene Cat#29573
pEGFP-Actin	(Mounier et al., 2009)	N/A
mCherry-Actin	Dominique Lallemand	N/A
pOrange-C3-Actin	(Ehsani et al., 2012)	N/A
pEGFP-Galectin-3	(Paz et al., 2010)	N/A
pOrange-Galectin-3	(Ray et al., 2010)	N/A
mTurquoise-Galectin-3	Noelia Lopez-Montero	N/A
pmCherry-CDC42	Sandrine Etienne-Manneville	N/A
pEYFP-CDC42	Joel Swanson	(Hoppe and Swanson, 2004)
GFP-CDC42-V12	Sandrine Etienne-Manneville	N/A
GFP-CDC42-N17	Sandrine Etienne-Manneville	N/A
pcDNA3-EGFP-RAC1	Klaus Hahn (Kraynov et al., 2000)	Addgene Cat#13719
pEYFP-RHOA	Joel Swanson	(Hoppe and Swanson, 2004)
pGFP-2XFYVE	Philippe Benaroch	N/A
pmCherry-2xFYVE	Harald Stenmark and Kay Oliver Schink	N/A
pEYFP-LAMP1	Walther Mothes	Addgene Cat#1816
pEGFP-LC3B	Thomas Wollert	N/A
pmCherry-RAB7	Michael Davidson	Addgene Cat#55127
Software and Algorithms
FIJI	NIH	https://fiji.schttps://imagej.net/ImageJ
Volocity 6.3 PerkinElmer	PerkinElmer	N/A
NIS-Elements Microscope Imaging Software	Nikon	N/A
GraphPad Prism	GraphPad Software v6.0, La Jolla, USA	https://www.graphpad.com/
Excel	Microsoft	N/A
ICY	Institut Pasteur	http://icy.bioimageanalysis.org/
Inkscape	Version 0.92.3	https://inkscape.org/
Other
Zeiss LSM780 Elyra PS1 microscope equipped with the followings:	Zeiss	N/A
63x/1.4 oil Plan Apo objective	Zeiss	N/A
EMCCD Andor Ixon 887 1K camera	Andor	N/A
Perkin Elmer UltraView spinning disk confocal microscope equipped with the followings:	Perkin Elmer	N/A
60 × /1.3 oil objective	Nikon	N/A
PSU C910-50 camera	Hamamatsu	N/A
Inverted widefield microscope equipped with the followings:	Nikon	N/A
20x/0.5NA air objective	Nikon	N/A
CoolSnap2 camera	Roeper Scientific	N/A
DeltaVision Elite epifluorescence microscope	GE Healthcare	N/A
96-well cell culture microplates with clear flat bottom	Greiner Bio One International	Cat#655090
Precision cover glasses thickness No. 1.5H	Marienfeld Superior	Cat#0117580
35 mm glass bottom μ-Dishes	Ibidi	Cat#81158
Tissue culture flask 75	TPP	Cat#90076
Costar® 6-well Clear TC-treated Multiple Well Plates	Corning	Cat#3516