FIGURE 3.

Expression of RSV genes and Stvb-i in seedling meristems. (A) Expression patterns of all seven genes present in the RSV genome (RdRp, P2, Pc2, NS3, CP, P4, and Pc4) in the cultivars Tsukinohikari (resistant) and Koganebare (susceptible). dai, days after inoculation. (B) Quantitative RT-PCR analysis of Stvb-i expression in Tsukinohikari. Transcript levels are shown relative to the level on day 0 (start of RSV inoculation), and are means ± SEM (n = 3). (C) Expression of Stvb-i and RSV NS3 in the Stvb-i–silenced transgenic T₂ lines St507 and St509. C10, non-inoculated plants of the same type at 10 dai were used as controls.