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. 2020 May 11;13(6):100775. doi: 10.1016/j.tranon.2020.100775

Figure 4.

Figure 4

231Br cells have higher migration and invasion potential compared to 231 cells. (A) Quantification of cells migrating across Transwells 72 hours after plating cells in the migration chambers measured by Transwell migration assay. Y-axis stands for the average number of cell migration per five microscope fields. All error bars represent SD, representative of two independent experiments. *P < .05, **P < .01, ***P < .001. (B) Quantification of cells migrating across Transwells 96 hours after plating cells in the Matrigel-coated migration chambers measured by Transwell invasion assay. Y-axis stands for the average number of cell migration per five microscope fields. All error bars represent SD, representative of two independent experiments. *P < .05, **P < .01, ***P < .001. (C) Quantification of cells presented in the scratch made on day 0 (0-hour time point) at 72 hours after AZA treatment by wound-healing assay. Cell numbers in the scratch wound were normalized to 0 hour. All error bars represent SD, representative of two independent experiments. *P < .05, **P < .01, ***P < .001. (D) Expression of MMP2, MMP9, vimentin, N-cadherin, and pan-cytokeratin in 231 and 231Br cells after AZA treatment for 72 hours measured by Western blotting assay. GAPDH was used as the loading control. The blots shown are a presentation of two independent experiments.