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. 2020 Apr 27;11:2041731420905701. doi: 10.1177/2041731420905701

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Sequential survival laparotomies to successively combine components of tissue-engineered small intestine: (a) Schematic illustration of the “double-surgery” procedure, followed by IVIS bioluminescence imaging and explanation. (b) Injection of ICG-tagged ENCCs into HIO-TESI during second survival operation. (c) Flow cytometry of ENCCs for CD271 (P75^NTR) and CD57 (HNK1) at day 15. Isotype control antibodies were applied. HIO: human intestinal organoid; ENCC: enteric neural crest cell; ICG: indocyanine green dye; HIO-TESI: tissue-engineered small intestine derived from human intestinal organoids; IVIS: in vivo imaging system.