Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 27;11:2041731420905701. doi: 10.1177/2041731420905701

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 5. — Human intestinal organoid–derived aganglionic tissue-engineered small intestine. Intestinal pacemaker cells ICC (CKIT+, a) and smooth muscle cells (SMA+, b) are identified in HIO-TESI with or without ENCC injection. Different cell types of epithelial lineage (ECAD+), including goblet cells (MUC2+, c), enteroendocrine cells (CHGA+, d), and Paneth cells (LYSO+, e), are present in HIO-TESI + saline and HIO-TESI + ENCC cysts. PCNA+ proliferating cells are located in the crypts and villus regions in the TESI (f). No OCT4 teratoma marker is observed (f). DAPI stained cell nuclei in all images. CKIT: ICC-selective receptor tyrosine kinase; SMA: alpha-smooth muscle actin; ECAD: E-cadherin; MUC2: mucin 2; CHGA: chromagranin A; LYSO: lysozyme; CALB: calbindin; CHAT: choline acetyltransferase; NOS1: neuronal nitric oxide synthase; 5-HT: serotonin/5-hydroxytryptamine; PGP9.5: protein gene product 9.5; PCNA: proliferating cell nuclear antigen; OCT4: octamer-binding transcription factor 4; hPSC: human pluripotent stem cell; ICC: interstitial cells of Cajal; HIO-TESI: tissue-engineered small intestine derived from human intestinal organoids.