Table 2.

Participants with L74I and longitudinal sampling

Participant	Subtype	Sample	ART line	Viral load (copies/mL)	pol drug resistance mutations (consensus sequence)	Integrase L74I (%)	Integrase L74I evolution
1	G	1	first	373 000	RT: M184V, K103N, Y318F	99.2	fixed majority
		2	first	274 500	RT: K70R, M184V, K219Q, K103N, Y318F	88.4
2	G	1	first	532 000	RT: D67N, K70R, M184V, T215I, K219E, Y181C	99.6	fixed majority
		2	second	36 000	RT: M184V, T215F, K219E, V179VLM, Y181C	90.9
3	G	1	first	29 000	RT: K70R, M184V, A98G, V108I, Y181C	95.6	fixed majority
		2	second	4300	RT: K70R, M184V, T215F, K219Q, V108I, Y181C, N348I	93.3
4	G	1	first	25 000	RT: M184V, K103N, K238T	99.5	fixed majority
		2	second	1 450 000	none	99.8
5	G	1	first	370 000	RT: M184V, K103N, Y318F	99.2	fixed majority
		2	second	275 000	RT: K70R, M184V, K219Q, K103N, Y318F	88.4
6	G	1	first	28 000	RT: M41L, D67N, T69D, K70R, V75M, M184V, T215F, K219Q, A98G, K103N, V179E, K238T, Y318F	85.5	fixed majority
		2	first	63 000	RT: M41L, D67N, T69D, K70R, V75M, M184V, T215F, K219Q, A98G, K103N, V179E, K238T, Y318F	99.1
		3	first	442 000	RT: M41L, D67N, T69D, K70R, V75M, M184V, T215FL, K219Q, A98G, K103N, V179E, K238T, Y318F	98.9
		4	second	5800	RT: M41L, D67N, T69D, V75M, M184V, T215IV, K219Q, A98G, K103N, V179E, K238T, Y318F	97.7
7	AG	1	first	48 000	RT: M184V, K103N, P225H	98.1	fixed majority
		2	second	11 000	RT: M184V, K103N	94.7
8	AG	1	first	241 000	RT: K103N, H221Y	99.5	fixed majority
		2	first	250 000	RT: Y181C, H221Y	99.0
		3	second	4400	RT: K103N, Y181C, H221Y	92.3
9	G	1	first	272 000	RT: K70E, Y115F, M184V, Y181C, G190A, H221Y	90.0	revertant majority
		2	second	24 000	RT: K70E, Y115F, M184V, Y181C, G190A, H221YPro: M46I, I54V, V82S, L24I, L33F	ND
10	G	1	first	59 000	RT: K65R, M184V, K219E, Y181C, H221Y	2.2	fixed minority
		2	second	1700	RT: K65R, M184V, K219E, Y181C, H221YPro: I54V, V82A, L10F	2.7
11	G	1	second	5700	RT: M41L, V75I, M184V, T215F, K101H, G190A, N348I	3.6	fixed minority
		2	second	384 000	RT: M41L, D67N, V75I, M184V, T215F, G190A, N348I	2.1
12	AG	1	second	5700	RT: M41L, V75I, M184V, T215F, K101H, G190A, N348I	3.6	fixed minority
		2	second	384 000	RT: M41L, D67N, V75I, T215F, G190A, N348I	2.1
13	G	1	first	20 000	RT: M184V, K101E, G190A	ND	emergent minority
		2	second	118 000	none	8.1
14	G	1	first	77 000	RT: M41L, E44D, D67N, K70R, L74V, M184V, T215Y, Y181C, G190A, N348IPro: L90M	39.2	revertant minority
		2	second	56 000	RT: M41L, E44D, D67N, T69D, K70R, L74V, M184V, T215Y, Y181C, G190A, N348IPro: M46I, 154V, V82A, L90M	2.4

RT, reverse transcriptase; Pro, protease; AG, CRF02_AG; ND, not detected.