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Effect of Pg-EE on the activation of signaling molecules in the NF-κB pathway. (A,B) Employing western blot analysis, the total and phosphorylated protein levels of inhibitor of kappa B alpha (IκBα), AKT, p85, Src, and β-actin were determined using whole cell lysates from LPS (1 μg/mL)-stimulated RAW264.7 cells with or without Pg-EE (150 μg/mL) in a time-dependent manner. −: no treatment and +: treatment.
