Figure 3.

RAMBO decreases endothelial cell activation. (A) RAMBO decreases endothelial cell activation. HUVEC or HDMEC cells were plated on 96-well plates and treated with conditioned media (CM) collected from mock-, rHSVQ-, or RAMBO-infected sarcoma cells. Eight hours post CM treatment, cells were treated with LeukoTracker^TM-labeled peripheral blood mononuclear cells (PBMCs) and allowed to adhere to endothelial cells. After 1 h incubation, unadherent PBMCs were washed off and adherent cells were lysed and quantified as instruction. Data is represented as relative fluorescence unit (RFU). (B) RAMBO decreases ICAM1 and VCAM1 gene expression. HUVEC and HDMEC cells were plated on 12-well plates. Six hours later, sarcoma cell lines were infected with 0.2 MOI of HSVQ and RAMBO for 30 min in a suspension condition. HUVEC and HDMC cells were overlaid with infected sarcoma cells and cultured for 24 h. Q-PCR analysis was conducted to examine for changes in gene expression of ICAM1 and VCAM1 between rHSVQ- and RAMBO-infected cells cocultured with endothelial cells. Data presented are fold changes in gene expression ± SD relative to GAPDH. * p < 0.05.