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. 2020 Mar 26;9(4):798. doi: 10.3390/cells9040798

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of tumor microenvironment cytokine gene expression changes due to virotherapy: Virotherapy-treated subcutaneous HapT1 (AIM2 −/− KO or “wt”) tumors were collected and analyzed by RTqPCR for indicated markers of the immunological status of the tumor. (A) IL1b, (B) IL2, (C) IL2Ra, (D) IL6, (E) IL10, (F) IL12p35, (G) INFg, (H) TNFa, and (I) CD83. Statistical significance towards the wt mock control was analyzed by Kruskal–Wallis test * p < 0.05; ** p < 0.01, *** p < 0.001, after ROUT outlier (Q = 1%) removal; lines represent median values.