Table 1.
In vitro assessment of intracellular virus-induced signaling in OAd.TNFa-IL2-infected SK-MEL-28 melanoma cells or dendritic cells (DCs), evaluated by RNA-sequencing: Top 8 genes showing the most marked upregulation in RNA expression levels as measured through RNA-sequencing (Poisson distribution filtering applied) are listed. Note that the virus codes for TNFa and IL-2 but transgene expression is linked to replication, which only occurs in tumor cells.
| SK-MEL-28 | ||||
|---|---|---|---|---|
| Gene | Abbreviation | Log2 Ratio (Infected versus Uninfected Cancer Cells) | Up- or Downregulation | p-Value |
| Interleukin 2 | IL-2 | 11.620 | Up | 0 |
| Tumor necrosis factor alfa | TNFa | 11.388 | Up | 0 |
| Ubiquitin | UBD | 9.958 | Up | 1.284006 × 10−182 |
| Histone H2A type 1 | HIST1H2AI | 9.935 | Up | 1.307986 × 10−08 |
| Chemokine (C-C motif) ligand 2 | CCL2 | 9.527 | Up | 6.97892 × 10 −71 |
| Abhydrolase domain containing 14A and aminoacylase 1 (read-through transcription) | ABHD14A-ACY1 | 8.991 | Up | 1.422174 × 1018 |
| Myotrophin | MTPN | 8.969 | Up | 1.681326 × 10−38 |
| Interleukin 32 | IL32 | 8.394 | Up | 9.93188 × 10−74 |
| DC | ||||
| Gene | Abbreviation |
Log2 Ratio (Infected versus
Uninfected DC) |
Up or Down-Regulation | p -Value |
| Myothrophin | MTPN | 9.629 | Up | 3.8971 × 10−65 |
| Locus101929802 | LOC101929802 | 6.931 | Up | 9.91084 × 10−14 |
| Locus102724994 | LOC102724994 | 6.919 | Up | 8.93212 × 10−07 |
| Zink finger816-Zink finger321P | ZNF816-ZNF321P | 6.443 | Up | 1.447082 × 10−05 |
| Heat shock 10kDa protein 1 and MOB family member 4, phocein (read-through transcription) | HSPE1-MOB4 | 5.807 | Up | 1.447082 × 10−05 |
| Nuclear Pore Complex Interacting Protein Family Member A3 | NPIPA3 | 4.518 | Up | 1.870752 × 10−16 |
| Chemokine (C-X-C motif) ligand 9 | CXCL9 | 3.0 | Up | 2.73386 × 10−10 |
| Platelet glycoprotein Ib alpha chain | GP1BA | 2.269 | Up | 7.3774 × 10−106 |