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. 2020 Apr 21;12(4):1021. doi: 10.3390/cancers12041021

Figure 1.

Figure 1

(A) Sections prepared from paraffin-embedded bone marrow (iliac crest) of patients with polycythemia vera (PV; patient #06), essential thrombocythemia (ET; patient #34) or primary myelofibrosis (PMF; patient #29) were stained with an anti-phosphorylated signal transducer and activator of transcription-5 (pSTAT5) antibody using immunohistochemistry. Examples of nuclear- and cytoplasmic staining are shown in Figure A1. Scale bar: 30 µm. Patient characteristics are shown in Table A1. (B,C) Bone marrow (BM) mononuclear cells (MNC) of patients with PV (patient #30), ET (patient #08) or PMF (patient #29) were stained with an anti-pSTAT5 Alexa-647 antibody. Intracellular expression levels of pSTAT5 were analyzed by flow cytometry in total MNC (B), or in cell subsets gated for CD34, CD14 or CD15 (C). The isotype-matched control antibody is also shown (open black histogram). Numbers in the small boxes represent the staining index defined as the ratio of the median fluorescence intensity (MFI) obtained with the anti-pSTAT5 antibody and MFI obtained with the isotype-matched control antibody (mIgG1).