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HEL and SET-2 cells were incubated with various concentrations of JAK2-targeting drugs (ruxolitinib, R763, TG101348, AZD1480) or STAT5-targeting drugs (piceatannol, pimozide, AC-3-019, AC-4-130) for 4 h. Thereafter, expression of pSTAT5 was analyzed by multicolor flow cytometry. The levels of pSTAT5 are depicted as median fluorescence index (MFI) values relative to the untreated control (Co).
