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Wild type (wt) Ba/F3 cells or Ba/F3 cells transfected with either JAK2 V617F or CALRdel52 were incubated overnight in medium without IL-3. After, cell lysates were subjected to Western blotting using antibodies against pSTAT5, STAT5 and HSC70. The columns show the levels of pSTAT5 in percent of HSC70 as analyzed by densitometry. Uncropped blots are shown in Figure S3.
