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. 2020 Apr 21;12(4):1021. doi: 10.3390/cancers12041021

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Figure A6 — To identify immature CD34⁺/CD38⁻ stem cells (SC), we performed a sequential gating algorithm, indicated by pink arrows using FlowJo software. After excluding CD45-negative cells, immature cells were identified by their expression of CD34 (upper left panel) and were then gated as CD45 dim-positive blast cells (lower left panel). Thereafter, viable cells were selected by their light-scatter properties (lower right panel) and were then gated according to the lack (pink gate) of CD38 (upper right panel). The example shows bone marrow cells from patient #23.