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. 2020 Apr 21;9(4):1024. doi: 10.3390/cells9041024

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ICAT overexpression inhibited the Wnt/β-catenin signaling targets, but upregulated protein levels of adipogenic regulators upon the CHIR99021 induction. (A) 3T3-L1 pre-adipocytes infected with lentivirus carrying ICAT. (B) 3T3-L1 pre-adipocytes stably overexpressing ICAT sorted by flow cytometry. (C) Protein expression for ICAT in stable overexpressing cells. (D) Protein expressions and (E) abundance analysis for adipogenic regulators and Wnt/β-catenin targets in 3T3-L1 adipocytes after 7 days differentiation. Values are means ± SEMs, n = 3 independent experiments. Means without a common letter differ, p < 0.05. C/EBPα, CCAAT-enhancer binding protein α; GSKi, CHIR99021; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAT, Inhibitor of β-catenin and TCF4; PPARγ, peroxisome proliferator activated receptor γ.