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. 2020 Apr 21;9(4):1024. doi: 10.3390/cells9041024

Figure 5.

Figure 5

ICAT or E2F1 knockdown inhibited MDI-induced differentiation of pre-adipocytes. (A) Protein levels for E2F1 and ICAT at 48 h after siRNA-mediated knockdown of E2F1. (B) Protein levels for ICAT and E2F1 at 48 h after siRNA-mediated knockdown of ICAT. (C) Protein abundances for adipogenic regulators and Wnt/β-catenin targets, and (D) TG contents of 3T3-L1 adipocytes after ICAT or E2F1 knockdown. (E) Representative micrographs of siE2F1 or siICAT transfected adipocytes after BODIPY493/503 (green) or oil red O (red) staining. (F) TG contents of stable E2F1 overexpressed 3T3-L1 adipocytes after ICAT knockdown. Values are means ± SEMs, n = 3 independent experiments. Means without a common letter differ, p < 0.05. C/EBPα, CCAAT-enhancer binding protein α; E2F1, E2 promoter binding factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAT, Inhibitor of β-catenin and TCF4; PPARγ, peroxisome proliferator activated receptor γ.