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. 2020 Apr 10;10(4):229. doi: 10.3390/brainsci10040229

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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NG2 expression in the corpus callosum at around 1 mm from bregma. (A–D) Fluorescence microscopy photographs show NG2+ oligodendrocyte progenitor cells in the corpus callosum. Double staining with Occludin (red) was done to distinguish NG2+ (green) vessel walls from oligodendrocyte progenitor cells (OPCs). DAPI+ nuclei are stained in blue. Arrows show NG2+/DAPI+/Occludin− OPCs, which appear scattered throughout the corpus callosum, with no apparent difference between sham and trauma-exposed subjects. Insert: the highlighted area refers to the area represented in (A–D). Scale bar = 50 µm.

NG2 expression in the corpus callosum at around 1 mm from bregma. (A–D) Fluorescence microscopy photographs show NG2+ oligodendrocyte progenitor cells in the corpus callosum. Double staining with Occludin (red) was done to distinguish NG2+ (green) vessel walls from oligodendrocyte progenitor cells (OPCs). DAPI+ nuclei are stained in blue. Arrows show NG2+/DAPI+/Occludin− OPCs, which appear scattered throughout the corpus callosum, with no apparent difference between sham and trauma-exposed subjects. Insert: the highlighted area refers to the area represented in (A–D). Scale bar = 50 µm.