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. 2020 Apr 10;12(4):933. doi: 10.3390/cancers12040933

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EpCAM-based recovery of HuP-T4, CAPAN-1 and MIA-PACA-2 cells spiked in mimicked-DLA products. (A) Recovery of 50 pre-labeled cells from the three lines using the IsoFlux system and three types of beads available from Fluxion (Iso-CEK, Iso-RCEK, and Iso-RCIK-SA) (upper panels) and using the KingFisher Duo system running the WuDuo1 program three different amounts (“MAX”, “MID” and “MIN”) of four types of beads available from Thermo Scientific (Dy-EpE, Dy-ACK, Dy-BioB, and Pi-Strep) (lower panels). (B) Recovery of different numbers of spiked pre-labeled HuP-T4 and CAPAN-1 cells using Iso-CEK and Iso-RECK-BerEP4 beads in the IsoFlux system, and Dy-EpE^MID and Dy-BioB^MAX-VU1D9 beads in the KingFisher Duo system.