Figure 4.

MUC-1 alone and MUC-1/EpCAM combined recovery of CAPAN-1 cells spiked in mimicked-DLA products. (A) (Left panel) Recovery of 50 pre-labeled CAPAN-1 cells with Iso-RCEK beads coupled with anti-MUC-1 clones EMA201 and GP1.4 alone or in combination (simultaneous) with anti-EpCAM coupled beads using the IsoFlux system. For the simultaneous MUC-1 and EpCAM recovery, half of the amount of each bead type was used, so that the total amount of beads in the experiment was according to the original protocol. Data in grey are the same as in Figure 2. (Right panel) Recovery of 50 pre-labeled CAPAN-1 cells with Dy-BioB beads coupled with the GP1.4 clone alone or in combination (simultaneously and sequentially) with Dy-BioB anti-EpCAM coupled beads using the KingFisher Duo system. For the simultaneous MUC-1 and EpCAM recovery, half of the amount of each bead type was used, so that the total amount of beads in the experiment was the same as described in the material and methods (Dy-BioB^MAX). Data in grey are the same as in Figure 2. (B) Recovery of 50 pre-labeled CAPAN-1 cells after sequential EpCAM- and MUC-1-based enrichment in the KingFisher system.