Table 2.

Resume of advantages and disadvantages found for both systems for CTC enumeration.

Isoflux
Advantages	Disadvantages
Easy to use Some flexibility concerning the type of beads	One single running modus Staining is done manually
KingFisher
Advantages	Disadvantages
Easy to use Inexpensive technology The running protocol can be customized and it can include multiple steps for enrichment with different epitopes, depletion of CD45 cells, and subsequent staining of samples Up to 96 samples can be run in parallel Flexibility concerning the type of beads Beads available combining high recovery and possibility of staining	Reagents are not provided as a kit Any change in the running protocol requires validation