Table 1.
Substrate scope of IacA and IacE. Products of the IacA-catalyzed reaction—homologs of Ox-IAA, products of IacE-catalyzed reaction—homologs of DOAA. “+” indicates successful conversion, “−“ indicates no conversion, NA—not analyzed. Bioconversion efficiency of IacA is presented as mean ± SD.
| Substrate | Structure | Activity | |
|---|---|---|---|
| IacA (Bioconversion Efficiency, %) | IacE | ||
| IAA |
|
+ (34 ± 8) | + |
| IPA |
|
+ (22 ± 5) | + |
| IBA |
|
+ (21 ± 5) | + |
| Indole |
|
+ (NA) | − |
| 3-(2-hydroxyethyl)indole |
|
+ (32 ± 7) | + |
| Ethyl-3-indole-acetate |
|
+ (19 ± 6) | + |
| 3-(3-hydroxypropyl)indole |
|
+ (37 ± 8) | + |
| 3-indoleacetonitrile |
|
+ (>90) | + |
| Indole-3-acetamide |
|
+ (28 ± 5) | − |
| Indole-3-lactic acid |
|
+ (43 ± 11) | − |
| Tryptamine |
|
− | NA |
| Indole-3-acrycil acid |
|
− | NA |
| Indole-3-carboxylic acid |
|
− | NA |