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. 2020 Mar 31;12(4):845. doi: 10.3390/cancers12040845

Figure 5.

Figure 5

The effect of ROS scavenging in plasma and PEF treatments. The plasma exposure time was 40 s, and the electric field strength was 0.9 kV/cm. The cells were pretreated with catalase (cat) or N-acetylcysteine (NAC) and stained with CellEvent Caspase-3/7 Green Detection Reagent at 24 h in the control condition (A), or after plasma treatment (B), PEF treatment (C), plasma plus PEF treatment (D), or PEF plus plasma treatment (E). The apoptotic cells (caspase 3/7 active cells) were quantified 24 h after the treatments (F), as well as the cell viability (G) and the metabolic activity (H). The significant differences of cells without any antioxidants compared to the cat and NAC pretreated cells were determined by t-test. Data are shown as one representative (A–E) or mean + S.E. (F–H) of three independent experiments. ctr = control; PL = plasma; PEF = pulsed electric fields; comb = combination; n.s. = not significant.