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. 2020 Mar 26;9(4):799. doi: 10.3390/cells9040799
AAVs Adeno-associated viruses
CRISPR/Cas9 Clustered regularly interspaced palindrome repeats (CRISPR)/Caspase 9 (Cas9)
crRNA CRISPR RNA
Crygc Crystalline gamma C
DOGS 5-carboxyspermylglycine dioctadecylamide
CaPO4 Calcium phosphate
cDNA Complementary DNA
ds Double-stranded
DSBs Double-strand breaks
dsRNA Double-stranded RNA
EEct Embryonic ectoderm
EGFP Enhanced green fluorescent protein
EP Electroporation
ExEct Extra-embryonic ectoderm
FITC Fluorescein isothiocyanate
Foxa2 Forkhead box protein A2
GE Genome-edited
GFP Green fluorescent protein
GONAD Genome-editing via oviductal nucleic acids delivery
gRNA Guide RNA
HDR Homology-directed repair
HR Homologous recombination
ICSI Intracytoplasmic sperm injection
i-GONAD Improved GONAD
IVF In vitro fertilization
KI Knock-in
KO Knock-out
NHEJ Nonhomologous end joining
ODNs Oligodeoxynucleotides
Otx2 Orthodenticle homeobox 2
PAM Protospacer adjacent motif
PGCs Primordial germ cells
rAAVs Recombinant AAVs
rAAV-6 Recombinant AAV serotype 6
RNP Ribonucleoprotein
SaCas9 Cas9 nuclease of the Staphylococcus aureus bacteria
sgRNA single guide RNA
Sry Sex-determining region Y
ss Single-stranded
TALENs Transcription activator-like effector nucleases
Tg Transgenic
TPGD Transplacental gene delivery
TPGD-GEF Transplacental gene delivery for acquiring genome-edited fetuses
tracrRNA Trans-activating CRISPR RNA
ZFNs Zinc-finger nucleases