AAVs |
Adeno-associated viruses |
CRISPR/Cas9 |
Clustered regularly interspaced palindrome repeats (CRISPR)/Caspase 9 (Cas9) |
crRNA |
CRISPR RNA |
Crygc |
Crystalline gamma C |
DOGS |
5-carboxyspermylglycine dioctadecylamide |
CaPO4
|
Calcium phosphate |
cDNA |
Complementary DNA |
ds |
Double-stranded |
DSBs |
Double-strand breaks |
dsRNA |
Double-stranded RNA |
EEct |
Embryonic ectoderm |
EGFP |
Enhanced green fluorescent protein |
EP |
Electroporation |
ExEct |
Extra-embryonic ectoderm |
FITC |
Fluorescein isothiocyanate |
Foxa2 |
Forkhead box protein A2 |
GE |
Genome-edited |
GFP |
Green fluorescent protein |
GONAD |
Genome-editing via oviductal nucleic acids delivery |
gRNA |
Guide RNA |
HDR |
Homology-directed repair |
HR |
Homologous recombination |
ICSI |
Intracytoplasmic sperm injection |
i-GONAD |
Improved GONAD |
IVF |
In vitro fertilization |
KI |
Knock-in |
KO |
Knock-out |
NHEJ |
Nonhomologous end joining |
ODNs |
Oligodeoxynucleotides |
Otx2 |
Orthodenticle homeobox 2 |
PAM |
Protospacer adjacent motif |
PGCs |
Primordial germ cells |
rAAVs |
Recombinant AAVs |
rAAV-6 |
Recombinant AAV serotype 6 |
RNP |
Ribonucleoprotein |
SaCas9 |
Cas9 nuclease of the Staphylococcus aureus bacteria |
sgRNA |
single guide RNA |
Sry |
Sex-determining region Y |
ss |
Single-stranded |
TALENs |
Transcription activator-like effector nucleases |
Tg |
Transgenic |
TPGD |
Transplacental gene delivery |
TPGD-GEF |
Transplacental gene delivery for acquiring genome-edited fetuses |
tracrRNA |
Trans-activating CRISPR RNA |
ZFNs |
Zinc-finger nucleases |