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. 2020 Apr 24;10(4):660. doi: 10.3390/biom10040660

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SAXS and SRCD analysis of Δ188AnxA11 and Δ192AnxA11. (A) SAXS data for Δ188AnxA11 and Δ192AnxA11 have been offset for clarity. GNOM, GASBOR, and CRYSOL fits are plotted over the measured data to denote the accuracy of distance distribution analysis, ab initio modeling, and theoretical scattering calculated from the crystal structure. (B) Guinier analysis. (C) Distance distribution diagram from GNOM. (D) Kratky plot of the SAXS data. (E) GASBOR ab initio models based on the SAXS data. The crystal structure (black) has been fitted inside. χ² values of the GASBOR and CRYSOL fits are indicated. (F) R_g as a function of CaCl₂ concentration. The error bars represent the fitting error from Guinier analysis. (G) SRCD spectra of Δ188AnxA11 and Δ192AnxA11 in the presence and absence of 1 mM CaCl₂.