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. 2020 Apr 18;9(4):1008. doi: 10.3390/cells9041008

Figure 4.

Figure 4

CB controlled both constitutive and LPS-activated interleukin expression in the middle stage of gestation. Comparison between mRNA profiles of key anti-inflammatory (IL-4 and IL-10) and pro-inflammatory interleukin (IL-12) recorded in middle stage AEC after 24 h of culture in the absence (constitutive) or presence of LPS stimulus (LPS-induced). Gene profiles were assessed by exposing the cells to 1 μM of selective CB1 or CB2 agonists (ACEA or JWH-015, respectively) or antagonists (AM281 or SR144282, respectively). Relative quantification was performed using the ΔΔCt method. GAPDH was used as housekeeping gene quantification. The data are mean ± SD of three independent replicates (n = 3 experimental replicates) performed using two different fetuses at middle stage of gestation (n = 2 biological replicates). Statistical analysis was performed using Student’s t-tail test. p < 0.05 was considered statistically significant (# values significantly different from CTR; a value statistically different between agonist and antagonist treatments).