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. 2020 Mar 25;12(4):772. doi: 10.3390/cancers12040772

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PAICS knockdown decreases the dissemination of CRC lesions to various organs. (A) Hematoxylin & eosin (H&E) staining of the hind-limb bones, lungs, and livers of mice injected with cells transfected with NT shRNA or PAICS shRNA. The images were taken at 4×and 20×; scale bar—20 µm. The arrows show metastatic lesions; BM, bone marrow; B, bone; Lu, lungs; Li, liver. (B) Immunofluorescence (IF) co-staining of liver with CDX2 as a marker of cancer cells (yellow) and arginase as a marker for liver cells; scale bar—50 µm. For bone staining, keratin 8 + 18 was used as a marker of cancer cells (yellow), ALP as a marker of osteoclast lineage (red), and DAPI for nuclear staining (blue); scale bar—20 µm.

PAICS knockdown decreases the dissemination of CRC lesions to various organs. (A) Hematoxylin & eosin (H&E) staining of the hind-limb bones, lungs, and livers of mice injected with cells transfected with NT shRNA or PAICS shRNA. The images were taken at 4×and 20×; scale bar—20 µm. The arrows show metastatic lesions; BM, bone marrow; B, bone; Lu, lungs; Li, liver. (B) Immunofluorescence (IF) co-staining of liver with CDX2 as a marker of cancer cells (yellow) and arginase as a marker for liver cells; scale bar—50 µm. For bone staining, keratin 8 + 18 was used as a marker of cancer cells (yellow), ALP as a marker of osteoclast lineage (red), and DAPI for nuclear staining (blue); scale bar—20 µm.