Figure 6.
PAICS initiates the epithelial mesenchymal transition (EMT) in CRC. (A) qRT-PCR analysis to evaluate mRNA expressions of PAICS, SNAI1, SLUG, ZEB1, and ZEB2 in HCT116p53-wt cells after PAICS knockdown. (B) The lysates of PAICS knockdown cells were blotted with antibodies for E-cadherin, vimentin, PAICS, or β-actin. The densitometric readings were calculated and normalized relative to the β-Actin. (C) Immunofluorescence for E-cadherin (red fluorescence) and DAPI (blue fluorescence) for nuclear stain in HCT116p53-wt cells with PAICS knockdown; scale bar—5 µm. (D) Immunoblot analysis for the expression of PAICS and E-cadherin in tumors excised from control mice or tumors treated with PAICS shRNA. The normalized ratio of the PAICS/β-Actin is reported. (E) Lysates of CRL1807 cells overexpressing adenovirus LacZ control and PAICS were used to check the expression of PAICS, E-cadherin, and vimentin.