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. 2020 Apr 2;12(4):860. doi: 10.3390/cancers12040860

Figure 5.

Figure 5

(A). Effects of CaSR inhibition on the expression of NE markers in PC3 and 22RV1. Cells were transfected with a siRNA control (siCTRL) or directed against CaSR (siCaSR) for 48 h (qPCR analysis were performed 48 h post-transfection). Results are normalized to control condition and are expressed as mean ± S.E.M. The statistical differences are indicated: ***p < 0.001 (Mann–Whitney). n = 3. (B). Immunohistochemical expression of CaSR and the neuronal marker NSE in 22RV1 cells, transfected with either a siRNA control (siCRTL) or directed against CaSR (siCaSR) for 48 h. CaSR inhibition is confirmed by an absence of immunostaining, and led to a decrease in NSE expression, from focal to almost diffuse.