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. 2020 Apr 23;9(4):1056. doi: 10.3390/cells9041056

Figure 4.

Figure 4

Modulation of miR-337-3p influences Twist1 and downstream thermogenic proteins. (a) RT-PCR analysis of mmu-miR-337-3p in murine brown adipocytes transfected with either precursor mmu-miR-337-3p or precursor scrambled molecule. (b) Western blot analysis of Twist-1 protein abundance of precursor mmu-miR-337-3p or precursor-scrambled transfected brown adipocytes and its quantification on the right (n = 2). (c) Western blot analysis of OXPHOS, Complex V-I, UCP1, and ACTB in brown adipocytes transfected with (left panel) either precursor mmu-miR-337-3p or precursor scrambled, or (right panel) transfected with LNA mmu-miR-337-3p inhibitor or scrambled inhibitor (n = 4 per group). (d) Quantification of OXPHOS Complex V-I protein abundance from (c) Western blot (e) Quantification of UCP1 protein abundance from (c) Western blot. Data are presented as means ± SEM, and individual data points are given by the dot plot. # p < 0.05, Independent sample t-test, equal variances not assumed * p < 0.05 Mann–Whitney-U for independent samples. ## p < 0.01, Independent sample t-test with equality of variances. n, number of biological replicas; OXPHOS, oxidative phosphorylation; UCP1, uncoupling protein 1; ACTB, beta-actin RT-PCR, quantitative real-time polymerase chain reaction.