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. 2020 Apr 15;12(4):978. doi: 10.3390/cancers12040978

Figure 2.

Figure 2

Cellular localization of heat shock protein 70 (HSP70) and high mobility group box 1 protein (HMGB1) on tumor cells treated with NB-PDT. Tumor cells were left untreated (NT) or treated with NB-PDT using 7D12-PS (LD50 or LD100), and 4 h later stained for HSP70 or HMGB1. Staining of HSP70 (red) was performed on non-permeabilized (a), A431 cells and (b), scc-U8 cells. Intracellular staining of HMGB1 (green) was performed on (c), A431 cells and (d), scc-U8 cells. Cell nuclei were additionally stained with DAPI (blue). Top panels depict only the damage-associated molecular pattern (DAMP) signal, while merged images are shown on the bottom panels. Scale bar, 20 µm.