Figure 5.

Expression of genes related to JAK–STAT and ligands for EGFR in the gut. Transcriptional levels of cytokine-inducing genes upd2 (A), upd3 (B), and the downstream target soc36 (C) were measured, as well as those for spi (D), krn (E), and vn (F). Flies were tested after six days after expression induction at 29 °C. Data represent mean values for 4 independent measurements (± SEM). Group comparisons were performed using ANOVA followed by Newman–Keuls post hoc test. Asterisks indicate significant difference from the control flies (esg/+) (p < 0.05); hash signs show significant difference from flies with inhibited IIS (esg/InR-RNAi).