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. 2020 Apr 22;9(4):1037. doi: 10.3390/cells9041037

Figure 2.

Figure 2

NF-κB subunit composition in early glutamatergic differentiation and c-REL potential function. (A) NF-κB subunit distribution in early stages of glutamatergic differentiation. Nuclear mean integrated density illustrated by fitting a polynomial function of the individual data normalized by the range of the median, showing each of the NF-κB subunits, as indicated by colors. (B) Quantified c-REL nuclear mean integrated density (n = 3, mean ± SEM). Nonparametric Mann Whitney test confirmed a significant decrease in nuclear c-REL after 5 days of differentiation with PTXF (*** p ≤ 0.0001), a known c-REL inhibitor. (C) 5 days differentiated NCSC-derived NSCs in the absence or presence of PTXF, labeled against c-REL shown by intensity scale and DNA colocalization. Arrows depict c-REL nuclear activation. (D) NCSC-derived NSCs differentiated in the absence or presence of PTXF for 30 days, labeled against cleaved-caspase-3 (cl.casp-3, intensity scale) showing DNA colocalization. Arrowheads depict dead cells. (E) Survival quantified for NCSC-derived NSCs differentiated in the absence or presence of PTXF for 30 days (n = 3, mean ± SEM). Nonparametric Mann Whitney test confirmed a significant decrease in survival PTXF-treated (*** p ≤ 0.001) compared to untreated neurons. PTXF: pentoxifylline, NCSCs: neural crest-derived stem cells, NSCs: neural stem cells.