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Validation of selected Wnt/β-catenin pathway by qRT-PCR in trabectedin-treated NCI-H295R cells. RNA was extracted and retro-transcribed as described in Materials and Methods. Genes were measured by q-RT-PCR using SYBR Green as fluorochrome. Results are presented as fold change ± SEM; * p < 0.01; ** p < 0.05; *** p < 0.001.
