Table 1.

Fatty acid methyl esters (FAME) (% relative quantities, %rel. quant.) obtained from membrane phospholipids of two different prostate cancer cell lines.

FAME 1	PC3 (n = 8) 2	LNCaP (n = 8) 2
C14:0	3.26 ± 0.32	3.44 ± 0.17
C16:0	33.20 ± 1.33	33.60 ± 0.49
6trans-C16:1	0.36 ± 0.04	0.56 ± 0.03 **
6cis-C16:1 n-10	7.41 ± 0.35	7.81 ± 0.33
9 cis-C16:1 n-7	2.36 ± 0.17	1.96 ± 0.05 *
C18:0	10.92 ± 0.54	11.48 ± 0.25
9trans-C18:1	0.09 ± 0.01	0.11 ± 0.02
8cis-C18:1 n-10	5.98 ± 0.75	4.55 ± 0.12
9cis-C18:1 n-9	19.47 ± 0.72	18.62 ± 0.24
11cis-C18:1 n-7	3.92 ± 0.30	3.90 ± 0.08
5cis,8cis-C18:2 n-10	0.47 ± 0.03	0.52 ± 0.04
mono-trans C18:2 n-6	0.27 ± 0.05	0.24 ± 0.02
C18:2 n-6	2.48 ± 0.19	1.97 ± 0.08 *
C20:3 n-6	1.62 ± 0.16	1.73 ± 0.18
C20:4 n-6	2.86 ± 0.23	4.01 ± 0.14 ***
mono-trans C20:4	0.10 ± 0.01	0.11 ± 0.05
C20:5 n-3	0.47 ± 0.06	0.37 ± 0.07
C22:5 n-3	1.77 ± 0.19	1.53 ± 0.14
C22:6 n-3	2.98 ± 0.18	3.49 ± 0.20
SFA	47.38 ± 1.39	48.52 ± 0.46
MUFA	39.14 ± 1.13	36.84 ± 0.59
PUFA	12.65 ± 0.37	13.62 ± 0.42
n-6	6.96 ± 0.34	7.70 ± 0.31
n-3	5.22 ± 0.15	5.40 ± 0.38
n-6/n-3	1.34 ± 0.07	1.52 ± 0.19
n-10	13.86 ± 0.97	12.89 ± 0.42
Total trans	0.83 ± 0.07	1.02 ± 0.06

¹ FAME = fatty acid methyl ester. Identified by standard references and quantified as described in the Materials and Methods Section. Values are obtained in µg/mL considering the gas chromatography (GC) peak areas recognized and calibrated with standard references (corresponding to >98% of the total peaks of the chromatogram). ² PC3, prostate cancer and LNCaP, Prostate; Derived From Metastatic Site: Left Supraclavicular Lymph Node. Values are expressed in percentages relative to the sum of all the quantities of the recognized peaks ± standard error of the mean (S.E.M) from the analyses of n = 8 cell samples of each type; statistical significance is estimated: * p-value ≤ 0.045; ** p-value ≤ 0.001; *** p-value ≤ 0.0009. Details of the statistical analysis are reported in the Materials and Methods Section.