Table 2.

Fatty acid methyl esters (FAME) (%rel. quant.) obtained from extracellular vesicles (EV) derived from two different prostate cancer cell lines.

FAME 1	PC3-EVs (n = 8) 2	LNCaP-EVs (n = 8)2
C14:0	4.38 ± 1.17	5.27 ± 0.25
C16:0	33.32 ± 0.74	35.62 ± 0.96
6trans-C16:1	0.10 ± 0.02	0.48 ± 0.03 ***
6cis-C16:1 n-10	6.10 ± 1.59	8.13 ± 0.28
9cis-C16:1 n-7	1.10 ± 0.26	1.59 ± 0.08
C18:0	12.35 ± 0.52	15.34 ± 0.66 **
9trans-C18:1	0.18 ± 0.04	0.22 ± 0.04
8cis-C18:1 n-10	2.85 ± 0.67	4.32 ± 0.27 *
9cis-C18:1 n-9	30.83 ± 4.41	15.43 ± 0.66 **
11cis-C18:1 n-7	1.21 ± 0.08	1.57 ± 0.24
5cis,8cis-C18:2 n-10	0.25 ± 0.03	0.62 ±0.07 ***
mono-trans C18:2 n-6	0.15 ± 0.03	0.20 ± 0.06
C18:2 n-6	3.43 ± 0.50	2.91 ± 0.18
C20:3 n-6	0.49 ± 0.08	0.98 ± 0.16 *
C20:4 n-6	0.70 ± 0.15	2.05 ± 0.13 ***
mono-trans C20:4	0.05 ± 0.02	0.08 ± 0.02
C20:5 n-3	0.29 ± 0.05	0.55 ± 0.09 *
C22:5 n-3	0.54 ± 0.14	1.41 ± 0.19 **
C22:6 n-3	1.68 ± 0.24	3.22 ± 0.23 ***
SFA	50.05 ± 1.50	56.23 ± 0.61 **
MUFA	42.08 ± 2.25	31.05 ± 0.49 ***
PUFA	7.39 ± 0.94	11.74 ± 0.41 ***
n-6	4.62 ± 0.57	5.94 ± 0.22 *
n-3	2.51 ± 0.38	5.18 ± 0.32 ***
n-6/n-3	1.97 ± 0.17	1.18 ± 0.08 ***
n-10	9.20 ± 2.23	13.07 ± 0.50
Total trans	0.48 ± 0.08	0.98 ± 0.09

¹ Identified by standard references and quantified as described in the Materials and Methods Section. Values are obtained in µg/mL considering the GC peak areas recognized and calibrated with standard references (corresponding to >98% of the total peaks of the chromatogram). ² EVs = extracellular vesicles. Values are expressed in percentages relative to the sum of all the quantities of the recognized peaks ± standard error of the mean (S.E.M) from the analyses of n = 8 cell samples of each type; statistical significance is estimated: * p-value ≤ 0.05; ** p-value ≤ 0.003; *** p-value ≤ 0.0006. Details of the statistical analysis are reported in the Materials and Methods Section.