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Transactivation activity assay of full-length and truncated IbWRKY2 in yeast. pGBK7+IbWRKY2, representing the full-length IbWRKY2, and pGBK7+N-IbWRKY2, representing IbWRKY2 with 246 aa removed from the N-terminal region, were transformed into the yeast strain AH109 and examined on SD/−Trp/−His selection medium with X-α-Gal. The pGBKT7 empty vector and pGAL4 were used as negative and positive controls, respectively.
